Applications and Articles - Testa Analytical Solutions

CHROMATOGRAPHY

NEWER ENTRIES

HPLC gradient validation using non-invasive flowmeters

Gradient HPLC serves as a crucial tool for analysing samples containing compounds with varying polarity. Its effectiveness stems from the ability to tailor both gradient time and composition, enabling the manipulation of analyte retention times. However, challenges arise from deviations in the programmed gradient profiles produced by HPLC systems, impacting retention time and peak shape. Several factors contribute to this variability in gradient HPLC, such as solvent quality, pump performance, changes in system pressure, and the condition of the columns. Addressing these challenges requires precise control and monitoring of gradient profiles.
Published in International Labmate – September 2024
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Validación de gradientes de HPLC con caudalímetros no invasivos

La HPLC de gradiente es una herramienta crucial para analizar muestras que contienen compuestos de polaridad variable. Su eficacia radica en la capacidad de adaptar tanto el tiempo como la composición del gradiente, lo que permite manipular los tiempos de retención de los analitos.
Published in Tecnicas de Laboratorio – July/August 2024
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Enhancing HPLC Field Service with fast-response, non-invasive flowmeters

Field Service Engineers are crucial to scientists striving to get the most from their analytical equipment. The maintenance, performance assurance and validation of HPLC/uHPLC systems gains particular importance when this equipment is an essential component in labs subject to compliance found in the pharmaceutical, food, beverage, and environmental analysis sectors.
Published in Lab Asia – June 2024
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Validating HPLC results obtained using any solvent

High-Performance Liquid Chromatography (HPLC) stands as a cornerstone technique in analytical chemistry, facilitating the separation, identification, and quantification of chemical and biological compounds. While many applications are based upon aqueous eluents, HPLC separation protocols also often involve the utilization of other solvents to accommodate the unique properties of different analytes. Ensuring consistent elution profiles is a key requirement to ensure reproducible results. Continuous monitoring of the delivered HPLC eluent flow rate is therefore essential when permanent validation of results is desired or necessary as it is in regulated industries.
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Preventing the ‘Jetstream effect’ in HPLC flow measurements

Determination of flow rate and comparison of the obtained results with expected or nominal values, is a very common task in liquid chromatography. It is also very often part of the validation of an HPLC/UHPLC system, as commonly required by labs following OQ/PQ guidelines. Among the methods available, modern non-invasive flowmeters are without a doubt the most flexible and capable tool for the task, as by nature they can deliver flow rate data with high accuracy and resolution in real time thus providing additional information as pulsation or short-term instability. As such, careful selection of a flow device used for the measurement is paramount for exploiting the full possibilities offered by such a powerful tool.
Published in MicroBioz India – May 2024
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HPLC Gradient Validation using Non-invasive Flowmeters

Enhancing HPLC Field Service with fast-response, non-invasive flowmeters

Field Service Engineers are crucial to scientists striving to get the most from their analytical equipment. The maintenance, performance assurance and validation of HPLC/UHPLC systems gains particular importance when this equipment is an essential component in labs subject to compliance found in the pharmaceutical, food, beverage, and environmental analysis sectors.
Published in MicroBioz India – April 2024
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Mejora del servicio de campo de HPLC con caudalímetros no invasivos y de respuesta rápida

Los ingenieros de servicio de campo son cruciales para los científicos que se esfuerzan por aprovechar al máximo sus equipos analíticos. El mantenimiento, aseguramiento del rendimiento y validación de los sistemas HPLC/UHPLC cobra especial importancia cuando estos equipos son un componente esencial en laboratorios sujetos a cumplimiento en los sectores farmacéutico.
Published in Laboratorio – April 2024
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Preventing the 'Jetstream effect' in HPLC flow measurements

The flow profile delivered by an HPLC system can be affected by an abrupt change in the internal diameter (ID) of tubing when the change is from a narrow bore to a significantly larger bore. Such an abrupt change in ID causes a "core stream" to be built in the larger ID tubing leaving the liquid layers closer to the wall of the tube practically unperturbed. This 'Jetstream effect' will decisively affect the velocity profile of eluant flow creating disturbances which will decrease the accuracy of HPLC flow rate measurement. Often underestimated, this phenomenon might also affect peak symmetry, it is therefore crucial to understand it and prevent.
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Laboratory 4.0 & Liquid Chromatography

In the world of analytical chemistry, liquid chromatography stands as a cornerstone technique, enabling scientists to separate, identify, and quantify components with unparalleled precision.
Published in MicroBioz India – February 2024
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Les partenariats technologiques favorisent l’innovation dans le domaine de la chromatographie liquide

Le développement de nouveaux produits visant à améliorer les capacités, les performances, la fiabilité, la facilité d’utilisation, la traçabilité des données et le service après‑vente est un objectif stratégique clé pour de nombreuses entreprises d’instrumentation.
Published in La Gazette du Laboratoire – February 2024
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Conformity or Calibration - which certification do you require for your Flowmeter?

All TESTA Analytical Flowmeters come with a Certificate of Conformity which demonstrates compliance of each individual product with our published specifications. The certificate of conformity testifies the internal process involved in assuring a certain quality of each and every flowmeter shipped to clients. This process includes fulfillment of a documented production process to a set of measurements aimed to prove that each and every instrument delivered will perform within specification.
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Technology partnering enables liquid chromatography innovation

Carlo Dessy is Managing Director of Testa Analytical Solutions eK, a respected international OEM developer and supplier of instruments, innovative detectors and software for liquid flow applications. Here I am sharing an interesting interaction with Carlo Dessy.
Published in MicroBioz India – November 2023
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Comparing techniques for flow rate measurement in Liquid Chromatography

Accurate determination of flow rate is one of the most common, and often overlooked, means of assessing or validating the performance of a pump serving a HPLC, UPLC, Ion-Chromatography or GPC/SEC system. Using the right technique, it is nowadays possible to use flow rate monitoring as a powerful diagnostic tool, allowing fast detection of leaks, faulty check-valves and worn seals.
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Studying the Impact of Dissolved and Undissolved Gas on the Performance of Peristaltic Pumps

A set of experiments illustrating how dissolved gas can affect peristaltic pumps in chromatography processes
Published in Lab Manager – July 2023
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Gefahr erkannt, Gefahr gebannt

Der Feind peristaltischer Pumpen sind Gasblasen. Welchen Einfluss gelöstes und ungelöstes Gas auf die Leistung dieser Pumpen hat und damit auf HPLC, GPC & Co. und was sich dagegen unternehmen lässt, erfahren Sie in diesem Beitrag.
Published in Laborpraxis – May-June 2023
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Studying the Impact of Dissolved and Undissolved Gas on the Performance of Peristaltic Pumps

Peristaltic pumps are fluid delivery tools widely used in laboratory techniques including HPLC, GPC/SEC, Flow Chemistry and Liquid Dosing. It is widely acknowledged that dissolved gas can have an impact on the performance of peristaltic pumps. A common solution to address this problem with HPLC systems, is to utilise a vacuum degasser on the inlet side of the pump.
Published in International LabMate – June 2023
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