MOLECULAR WEIGHT DETERMINATION
Comparing Goniometer-based & enclosed Light Scattering instruments
At first sight, goniometer based and enclosed light scattering instruments look very different in terms of the bench space they occupy. In this technical article we look to underline some of the key details and differences between these two approaches to Dynamic Light Scattering (DLS) which might not be immediately obvious to someone interested in learning more about the topic.
Published in Microbioz India – June 2023
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Using Static Light Scattering to Measure Polymer Molecular Weights
Static light scattering (SLS) is a technique that leverages high precision photon counting to measure size, molecular weight, and other structural parameters. Common samples include synthetic polymers, nanoparticles, biomolecules, and other macromolecules...
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Why is Flow Rate Important in GPC/SEC?
To obtain reliable data from your GPC/SEC instrument - each run should be related to a calibration curve obtained by measuring appropriate chromatographic standards. Calculations from GPC/SEC experiments are based on elution volume, which is a product of time and flow rate...
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Static Light Scattering of Biomacromolecules
Static Light Scattering (SLS) is capable of measuring the average molecular weight of polydisperse materials, as well as of samples with discrete aggregation numbers, such as proteins. Synthetic polymers are intrinsically polydisperse and are well-suited to direct analysis using this technique, thus the meaning of an average molecular weight is more direct...
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How Enhanced Detector Stability Leads to Improved GPC/SEC Results
GPC (Gel Permeation Chromatography) or SEC (Size-Exclusion-Chromatography is widely acknowledged as one of the most useful characterization methods in polymer science. The technique offers the possibility to determine molecular weight distribution and its averages, to provide a complete picture of the polymer sample under investigation...
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Light Scattering: a key Tool for Performance Polymer Characterisation
The majority of modern performance polymers are designed to enhance a particular material property. A number of high-profile engineering plastics, resins, and gels are made from these kinds of precursors. Light scattering can be used to solve many of the unique problems encountered when working with unusual functional plastics and associated polymeric precursors...
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High Performance GPC/SEC System
Gel-permeation chromatography (GPC) coupled with Multi-Angle Static Light Scattering (SLS) is a modern and powerful technique for determining absolute molecular weight values without the need to rely on traditional column calibration...
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Determination of specific refractive index increment (dn/dc)
The specific refractive index increment (dn/dc) is a fundamental parameter for the determination of molecular weight with Static Light Scattering...
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Common detectors for GPC/SEC Chromatography
This on-line presentation provides an overview about the detectors most commonly used in GPC/SEC applications, explains differencies...
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