Gel-permeation chromatography (GPC) coupled with Multi-Angle Static Light Scattering (SLS) is a modern and powerful technique for determining absolute molecular weight values without the need to rely on traditional column calibration. In GPC- MALS, the signal intensity levels are evaluated quantitatively rather than by elution times. Furthermore, unlike polymer standard calibration, details of the accurate sample concentration, injection volume and corresponding mass are required for accurate interpretation of light scattering signals.
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