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GPC/SEC Chromatography GPC/SEC Chromatography


The AYE Combo Viscometer - Refractometer sets a new standard for the detection of intrinsic viscosity of highly diluted samples. The Refractometer is integral part of the viscometer, therefore both concentration and viscosity are measured at exactly the same time on the very same sample segment. The AYE Combo allows a much more accurate determination of molecular parameters and is particular suited for those application focused on the structure and branching of the investigated polymer.

Differential Viscometer - Refractive Index GPC/SEC detector combo

Quickly, easily, and accurately determine absolute molecular weights of proteins and polymers. Eliminate SEC/GPC column calibration and improve data quality. Rapidly reduce uncertainty with no assumptions required and true extrapolation to zero angle. Best of all, the BI-MwA has the highest performance/price ratio of any light scattering detector used for molecular weight determination.

BI-MwA Mutli-Angle Light Scattering detector (MALS)

Within a GPC/SEC Chromatography system, a viscometer allows use of a universal calibration. Molecular weights obtained with this method are therefore independent of the polymer standards used to build the calibration and can be considered to be close to absolute values. Also, a viscometer will allow determination of further molecular parameters giving insight about the physical structure of the investigated polymer and even opening the gate to determination of branching parameters. All viscometers for GPC/SEC currently on the market, including the AYE Viscometer, base their principle on the measurement of the differential pressure across a capillary network. TESTA has been able to dramatically reduce the noise level and increase sensitivity by more than an order of magnitude compared to any viscometer available. This allows usage at much lower flow rates than it has been possible. Detection of lower molecular weight and at lower concentration are now also possible. Semi-micro applications are currently easily reached.

Differential Viscometer GPC/SEC detector

GPC/SEC Chromatography with MALS Detector is a very powerful tool capable of delivering absolute values of the compound under investigation. To achieve this, it is absolutely necessary to obtain concentration data along with the molar mass. In this scenario, a common DRI will lack the capability to deliver data measured at the same wavelength as the laser used in the MALS Detector. This fact will introduce a non-neglectable error in all calculations performed, thus questioning the obtained results. The AYE DRI operates at the very same wavelength your MALS Detector does, thus improving reliability of your results.

Differential Refractive index GPC/SEC detector

UV Detectors provide unsurpassed sensitivity and selectivity as concentration detectors in GPC/SEC. The only limitation is that the sample must contain chromophoric groups and that the solvent used provide a window in the absorption range of UV light. With these conditions fulfilled, a UV Detector is of great use as a single concentration detector in GPC/SEC or, combined with a DRI Detector, for the determination of composition distribution of copolymers. The AYE Rapid Scanning UV combines high sensitivity, a huge linear range of more that 4 orders of magnitude and fl exibility in the application, with solid physical base of clearly defined bandwidth and wavelength precision. It allows detection at two wavelengths, optional four wavelengths, at the same time and is therefore best suited for application where complex copolymers need to be characterized.

Rapid Scanning Multi Wavelengths UV detector

ParSEC is a powerful new suite of software for multi detector, macromolecular characterization and represents the most significant development in GPC/SEC analysis. ParSEC is compatible with ALL GPC/SEC systems and detectors. Many attractive and practical features have been written into the new software for the benefit of chromatographers, including "function specific" views, ensuring the uncluttered display of information relevant to the task at hand, customizable display and annotation options for all graph types, plus a Database approach for practical storage and archiving of data and results associated with any application.

ParSEC advanced GPC/SEC software

GPC/SEC Chromatography is impossible without Polymer Standards. For QC Applications which mostly uses a single concentration detector, Polymer Standards are required for Calibration of the Columns which then allows calculation of Molecular Weights. More complex GPC/SEC Systems with Viscometers and MALS, require Polymer Standards for the determination of Instrument Constants and for System Validation. These are the facts which make GPC/SEC without Polymer Standards impossible. A ReadyCal™ Kit combines highest quality standards with easy and fast handling, reducing errors in sample preparation practically to zero. Three sets each of four different pre-mixed standards in 1.5 mL screw cap Vials build the strong backbone of a reliable calibration curve. Just add the desired amount of solvent, shake according to instruction and inject. Column calibration cannot be easier.