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⇤ APPLICATIONS, ARTICLES & TECHNICAL NOTES

Why is Flow Rate Important in GPC/SEC?

Why is Flow Rate Important in GPC/SEC?

To obtain reliable data from your GPC/SEC instrument - each run should be related to a calibration curve obtained by measuring appropriate chromatographic standards. Calculations from GPC/SEC experiments are based on elution volume, which is a product of time and flow rate. As such, knowledge of GPC/SEC flow rate is of paramount importance as it is required to be constant over much longer periods of time than with HPLC separations.