July 21st 2021
Quantifying the amount of protein is highly desirable before isolating, separating and analysing samples using chromatographic techniques. Refractive index (RI) is one of the most commonly used detectors in GPC/SEC and HPLC systems. A key parameter for translating RI detector output to exact sample concentration is the dn/dc value, or specific refractive index increment. The HK Series Differential Refractive Index (DRI) detector from TESTA Analytical has been shown to provide accurate and reliable measurement of the absolute concentration and total mass of protein in a sample.
July 14th 2021
A new application note demonstrates how Static Light Scattering (SLS) is capable of measuring the average molecular weight of polydisperse materials, as well as of samples with discrete aggregation numbers, such as proteins. When studying Bovine Serum Albumin (BSA), a protein known to exhibit three isoforms (Mw = 66.5 kDa, 133 kDa, and 200 kDa), the meaning of average molecular weight can be less obvious. The nature of this relationship is demonstrated by comparing the molecular weight measured on our BI-200SM goniometer using traditional static light scattering to the relative abundances from a size exclusion chromatography (SEC) experiment.
July 7th 2021
When a liquid chromatography manufacturer evaluates whether to develop in-house (make) or partner with an experienced external company (buy) to create a new speciality detector – performance, whilst important, it not the only decision factor to be considered. A new paper is available that discusses the three fundamental factors (know-how, resources and time) that affect this decision.
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