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  • Screening for Protein Aggregation...Read More

  • Fast & Flexible Nanoparticle Size Analyser...Read More

  • High Performance Refractive Index Detector ...Read More

  • Automatic Continuous Online Monitoring of Polymerization Reactions...Read More

  • Dynamic Light Scattering Measurement in High Concentration Solutions...Read More

  • Assessing Nanoparticle Antimicrobial Properties...Read More

  • Zeta Potential Measurement on Scarce, Concentrated or Sensitive Samples...Read More

  • Quantitative, High Resolution X-Ray Particle Sizing...Read More

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    Screening for Protein Aggregation

    Testa Analytical Solutions reports on considerable interest from protein scientists to use Dynamic Light Scattering (DLS) as a tool for screening for the presence of aggregates as it is extremely sensitive to these larger compounds.

    Applications Lab Manager - Raffaele Carano said "Protein aggregation is a common challenge in the manufacturing of biological products. Protein scientists particularly like DLS because it provides them with a simple, fast and non-destructive test for aggregate detection, where the very same sample can also be used for Small-Angle X-ray Scattering (SAXS) or Small-Angle Neutron Scattering (SANS) experiments".

    He added "SAXS or SANS are powerful techniques used to extract structural parameters and determine the overall structures and shapes of biological macromolecules, complexes and assemblies in solution. The presence of aggregates is known to negatively affect SAXS and SANS results, consequently scientists can use DLS as a selection tool to make sure protein samples are "good" candidates for their measurements".

    The NanoBrook 90Plus particle size analyzer incorporates all you need for fast, routine, sub-micron measurements. Based on the principles of Dynamic Light Scattering (DLS), most measurements only take a minute or two. Protein aggregation in samples can occur due to changes in pH or temperature. The NanoBrook 90Plus in association with an autotitrator is a perfect system for monitoring protein samples for aggregation.

    For further information on this application please contact Testa Analytical Solutions on +49-30-864-24076 / info@testa-analytical.com.

     

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    Fast & Flexible Nanoparticle Size Analyser

    If you have polymers or proteins, nanodots or new adhesives, latexes or liposomes, cosmetics or colloids, the Testa Analytical NanoBrook 90Plus is a perfect tool to characterize your particles.

    The 90Plus is designed to perform fast, routine submicron particle size measurements (<1 nm to 6 µm) on a wide variety of samples and concentrations. Based on the principles of dynamic light scattering, most measurements only take a minute or two.

    Using simple to use but powerful tools the 90Plus is able to extract the most information from your sample. A unique filter algorithm can remove the influence of a contaminating fraction of large dust particles. Such particles can ruin a light scattering measurement; with the 90Plus it is often possible to rescue the measurement and obtain reliable data. The flexible and intuitive software allows you to quickly, simply, and interactively test the effects of changing parameters and evaluation methods. Different levels of software operation both enable a novice or occasional user to obtain key data quickly while still providing access to advanced features enough for specialists to unlock valuable data from complex measurements.

    Another important benefit for users of the 90Plus is its outstanding reproducibility from sample-to-sample, operator-to-operator, and instrument-to-instrument. The high-quality optical design and strict quality control during manufacture ensures that every instrument performs the same. By automating much of the testing procedure and data analysis, effects due to different operators are dramatically reduced.

    Besides particle sizing, the 90Plus is also upgradeable to perform zeta potential measurements to enable you to better understand colloidal behaviour.

    For further information on the NanoBrook 90Plus please visit https://www.testa-analytical.com/index.html?dc=Particle&sn=4 or contact Testa Analytical Solutions on +49-30-864-24076 / info@testa-analytical.com.

     

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    High Performance Refractive Index Detector

    Testa Analytical Solutions e.K is now offering optimized versions of its proprietary Differential Refractive Index (DRI) Detector under OEM contract to enhance the performance of both HPLC and GPC/SEC systems.

    Carlo Dessy, Managing Director of Testa Analytical said "Our compact DRI Detector and DRI Detector Kits offer a wide range of options in terms of Light Source, Electronics and Firmware solutions. This means it can be readily adapted to fit the requirements of any third party HPLC or GPC/SEC system. Our DRI detector will improve the accuracy and reliability of your Chromatography system when determining absolute concentration and total mass balance. Operating from room temperature up to 80 °C with an outstanding thermal stability, the DRI detector also offers unsurpassed baseline stability and fast setting. Its sensitivity makes it the perfect RI detector to integrate into modern GPC/SEC and HPLC systems and to achieve peak performance even in the most challenging applications".

    He added "The flexibility of our detector design has put us in the position to supply OEM versions in a wide range of requirements including low flow rate (down to 300 µL/min) as well as more classical HPLC application such as detection of sugars. Beneficially we are able to offer these OEM Solutions starting at very low yearly volumes, which makes our DRI detector the perfect match also for company's focusing on niche chromatography applications."

    For further information on the DRI Detector please click here. To discuss integrating an optimized version of the DRI detector into your GPC/SEC or HPLC system please contact Testa Analytical Solutions on +49-30-864-24076 / info@testa-analytical.com.

     

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    Automatic Continuous Online Monitoring of Polymerization Reactions

    Testa Analytical Solutions e.K reports on the development and supply of key detector components for ACOMP, a smart manufacturing system by Fluence Analytics that continuously analyzes polymers during production.

    ACOMP is an automated monitoring system that produces real-time data about reaction kinetics and polymer properties, including residual monomer, monomer conversion, polymer composition, molecular weight and intrinsic viscosity.

    Carlo Dessy, Managing Director of Testa Analytical said, "Fluence Analytics is a pioneering developer of novel analytics solutions for optimizing the efficiency and quality of polymer and biopharmaceutical production. Fluence Analytics selected us to to develop rapid scanning UV and differential refractive index detectors for ACOMP. Unlike many OEM projects we have worked on, this was not a chromatographic application. There is no separation involved. With the requirement for detection at higher flow rates, we had to adapt our standard UV and differential refractive index detectors. We also had to write new software for this polymerization monitoring application."

    "TESTA Analytical is a highly respected manufacturer of refractive index and UV detectors with an impressive record of designing innovative solutions to target challenging applications. TESTA Analytical's support has enabled Fluence Analytics to help customers increase polymer yields, quality and consistency; optimize process control; and reduce cycle times and material usage," said Alex Reed, CEO of Fluence Analytics. For further information on the ACOMP, visit https://fluenceanalytics.com/acomp/.

     

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    Dynamic Light Scattering Measurement in High Concentration Solutions

    Testa Analytical Solutions e.K announces a comprehensive new applications study that explains why making Dynamic Light Scattering (DLS) measurements in concentrated solutions is difficult.

    Dynamic Light Scattering is a widely used and effective technique for measuring the hydrodynamic size of common materials including colloids, nanoparticles, proteins, and polymers.

    Despite the versatility of this technique, the study discusses two major factors that impact the ability to measure particle size at high concentrations: multiple scattering and interparticle interactions.

    The authors of the study conclude that even in the absence of multiple scattering, measurements made in the semi-dilute region do not necessarily reveal particle size, and thus great care must be used to interpret such results. It is also demonstrated that serial dilution can be used to identify the region in which the sample is sufficiently dilute to reliably calculate particle size directly.

    The NanoBrook Omni particle sizer and zeta potential analyzer from Testa Analytical provides users with fast, routine, sub-micron measurements of size and zeta potential for proteins, polymers and colloidal dispersion applications. Based on the principles of Dynamic Light Scattering (DLS) for particle sizing and distribution, and on Doppler velocimetry (electrophoretic light scattering, ELS) for zeta potential, most measurements only take a minute or two. The instrument also includes Phase Analysis Light Scattering (PALS) measurements for samples with low mobilities such as saline and PBS.

    For a copy of the new application study please visit www.testa-analytical.com/index.html?dc=Knowledge&sn=18. For further information on the NanoBrook Omni please visit www.testa-analytical.com/index.html?dc=Particle&sn=1 or contact Testa Analytical Solutions on +49-30-864-24076 / info@testa-analytical.com.

     

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    Assessing Nanoparticle Antimicrobial Properties

    Testa Analytical Solutions e.K. reports on how a BI-200SM light scattering goniometer has been used to help assess the antimicrobial properties of silver and copper nanoparticles.

    In the study, various strains of Escherichia coli, Bacillus subtilis and Staphylococcus Aureus were treated with suspensions of suspended silver and copper nanoparticles. To assess the effectiveness of the nanoparticles, a BI-200SM goniometer was used to measure the size of the suspended nanoparticles by obtaining particle size distribution data via dynamic light scattering (DLS).

    Carlo Dessy, Managing Director of Testa Analytical commented "Silver and copper ions have been known to have antimicrobial properties and are used as superior disinfectants for wastewater generated from hospitals containing infectious microorganisms. Learning how to manipulate these silver and copper nanoparticles can lead to discoveries that may greatly impact applications in the fields of medical devices, food processing, and water treatment". He added " I am very pleased to see that the BI-200SM goniometer has helped the researchers that undertook this study to conclude "silver and copper nanoparticles show great promise as antimicrobial agents against Escherichia coli, Bacillus subtilis and Staphylococcus Aureus."

    The Bl-200SM goniometer system is a precision research grade instrument designed for exacting light scattering measurements. Based on a special turntable with precision ball bearings and stepping motor, the Bl-200SM's modular, automated design and quality construction guarantee precise measurements due to the wobble-free movement of the detector. As you would expect from a research grade system the BI-200SM can measure light scattering over a wide angular range (8° to 155° with 25 mm cells) and offers fine adjustment of measurement angles to 0.01° directly using a large, fine-control knob. Fine-screw vertical adjustment makes centre of rotation measurement easier when aligning cells. Precise repeatable data is ensured by automated heating and cooling of the sample cell using and standard external recirculating system. Field proven in hundreds of labs around the world the BI-200SM light scattering goniometer system is ideal for even the most demanding macromolecular studies and submicron particle sizing applications.

    For a full copy of this study please visit www.testa-analytical.com/index.html?dc=Knowledge&sn=17. For further information on the BI-200SM research goniometer system please visit www.testa-analytical.com/index.html?dc=Scattering&sn=1 or contact Testa Analytical Solutions on +49-30-864-24076 / info@testa-analytical.com.

     

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    Zeta Potential Measurement on Scarce, Concentrated or Sensitive Samples

    Testa Analytical Solutions e.K. has introduced a Small Volume Electrode (BI-SVE175) that pushes the boundaries of Zeta Potential measurement. Its novel design minimizes waste and preserves expensive or scarce samples with its ability to measure Zeta Potential from a sample volume of just 175 microliters.

    Not only does the BI-SVE175 preserve samples, it can measure sensitive samples such as proteins without denaturing them. In addition, its use of low voltage allows samples with extremely high salt concentration to be measured without difficulty. 

    The BI-SVE175 small volume  electrode has been designed to operate optimally with Testa Analytical's NanoBrook series of Zeta Potential instruments.

    Although it is used in the same way as a standard electrode cell on NanoBrook series instruments, the BI-SVE175 enables accurate and repeatable Zeta Potential measurements in highly concentrated samples. By requiring less sample dilution, using the BI-SVE175 more closely preserves the pH and chemical structure of your sample, thereby producing more directly representative measurements.

    This capability is particularly beneficial for many colloidal materials, where measuring the sample in its native state avoids the need for the often complicated centrifugation and dilution procedures needed to maintain sample integrity.

    To watch a video demonstrating how the BI-SVE175 small volume electrode can enhance your zeta potential measurements please visit https://www.testa-analytical.com/index.html?dc=Zeta&sn=5. For further information please contact Testa Analytical Solutions on +49-30-864-24076 / .

     


    Quantitative, High Resolution X-Ray Particle Sizing

    Testa Analytical Solutions e.K announce its BI-XDC X-Ray Disc Centrifuge is enabling scientists to make accurate, high resolution particle size distribution measurements, from nanometres to tens of micrometres, without time consuming calibration or absorption correction.

    Based on the principle of photosedimentation, the BI-XDC measures the size of particles according to the time the particle takes to sediment in the detector according to Stokes law. Combining centrifugal and gravitational sedimentation in a single instrument the BI-XDC sets a new standard for these well-established methods of particle sizing.

    Leveraging a highly reliable orthogonal method, the BI-XDC is proven to give error free quantitative measurements across a broad range of samples including nanoparticles, minerals, clays, ceramics and metal oxides.

    Using the Bl-XDC there are no optical corrections and no optical properties to worry about, just a simple mass sensitive response based on x-ray absorption.  The BI-XDC features a reproducible digitally controlled disk speed to ensure high performance and accuracy. The proprietary scanning x-ray head enables measurements to be made typically in just 8 minutes.

    For further information on the BI-XDC X-Ray Disc Centrifuge please visit www.testa-analytical.com/index.html?dc=Particle&sn=6 or contact Testa Analytical Solutions on +49-30-864-24076 / info@testa-analytical.com.

     

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    Light Scattering Goniometer Reveals Why Molybdenum Solutions Are Blue

    Testa Analytical Solutions e.K reports on how a BI-200SM light scattering goniometer system with autocorrelator has helped researchers at a prestigious government laboratory to solve the enigma of why aqueous molybdenum solutions are blue.

    A spokesperson from the Department of Physics at the government laboratory said "Molybdenum and oxygen can combine to form a wide variety of structures, and some polyoxomolybdate (POM) molecules are the biggest inorganic molecules known, achieving similar sizes to proteins". He added "The electronic state of the POMs is responsible for imparting a blue color to the surrounding solution, as the giant POMs cluster together rather than existing as single ions. Because of the size of the POMs, we realized we could use our BI-200SM light scattering goniometer to find out the actual structure of the complexes and we subsequently discovered a unique blackberry arrangement that we believe represents a new, stable solute state. It has changed our understanding of the solution behavior of inorganic molecules when they reach nanometer sizes."

    The Bl-200SM goniometer system is a precision research grade instrument designed for exacting light scattering measurements. Based on a special turntable with precision ball bearings and stepping motor, the Bl-200SM's modular, automated design and quality construction guarantee precise measurements due to the wobble-free movement of the detector. As you would expect from a research grade system the BI-200SM can measure light scattering over a wide angular range (8° to 155° with 25 mm cells) and offers fine adjustment of measurement angles to 0.01° directly using a large, fine-control knob. Fine-screw vertical adjustment makes centre of rotation measurement easier when aligning cells. Precise repeatable data is ensured by automated heating and cooling of the sample cell using and standard external recirculating system. Field proven in hundreds of labs around the world the BI-200SM light scattering goniometer system is ideal for even the most demanding macromolecular studies and submicron particle sizing applications.

    For further information on the BI-200SM research goniometer system please visit www.testa-analytical.com/index.html?dc=Scattering&sn=1 or contact Testa Analytical Solutions on +49-30-864-24076 / info@testa-analytical.com.

     

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    Aggregation Detection by Dynamic Light Scattering

    Testa Analytical Solutions e.K reports on how its NanoBrook Omni particle size analyser and BI-ZTU autotitrator can be used together to detect the onset of aggregation in nanoparticles.

    The NanoBrook Omni instrument offers a single powerful and accurate solution for particle sizing and zeta potential analysis. The instrument allows users to measure samples in nearly every possible suspension environment from high salts such as PBS and sea water to organic solvents and aqueous solutions. The instrument offers unmatched particle sizing, with the highest sensitivity, utilizing three light scattering measurement angles for truly unbiased measurement results. The BI-ZTU autotitrator option for the NanoBrook Omni provides an ideal solution for automated measurement of particle size as a function of pH.

    Applications that use iron oxide nanoparticles are vast and growing due to its stability, low toxicity, and magnetic properties. Given the great potential for applications using iron oxide, it is highly desirable to understand the effect of chemical changes to the surface of the nanoparticle.

    Aggregation of nanoparticles can occur due to changes in pH or temperature. In a new applications report* researchers show how Dynamic light Scattering (DLS) offers a quick and easy way of determining the effective diameter (or size) of iron oxide nanoparticles and an autotitrator can help automate the process of detecting changes in particle size as a function of pH. They conclude that the NanoBrook Omni particle size analyser and BI-ZTU autotitrator provide a highly effective instrumental solution for the detection on aggregation of nanoparticles due to changes in pH.

    For a copy of the application report please go to www.testa-analytical.com/index.html?dc=Knowledge&sn=14. For further information on the NanoBrook Omni instrument please visit www.testa-analytical.com/index.html?dc=Zeta&sn=1 or contact Testa Analytical Solutions on +49-30-864-24076 / info@testa-analytical.com.

     

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